[[abstract]]Most of us are used to using animals in our life. The issue of animal’s welfare is always discussing around us. When the 99 biology curriculum outline was formulated, the association of animal protection suggested that the frog-dissecting experiment would be cancelled to decrease sacrified animals and to reduce students’ learning confusion. The main focus of this study was to investigate the attitude about animal-using of second grade students in senior high. Self-compiled questionnaire, the attitude questionnaire about animal using, was the study implement. By judgment sampling, the sending-out questionnaire totaled 596 and sampled from second grade students with majoring in science of national senior high in Taipei County, Taipei City, and Keelung City. The valid questionnaires were 576, including 292 males and 284 females.The data was analyzed by descriptive statistics, independent-samples t test, and one-way ANOVA. The result revealed that total students’ attitude tended to positive (agreeing to use animal), and there was significant difference in gender. Other background conditions (school types, experience of experiment, academic groups in third grade, major subject for college, and religion) didn’t show significant differences, but sub-scales presented some differences. Via data collecting, some students expressed the respecting-life concept and caring about animal’s welfare. Based on the results, biological teachers should emphasize the importance of respecting life and using animal with humanity. And the superintendent of education should hole some workshops or develop some substitutes for animal to increase choise of teachers and students. Key words : bioethics, animal use, attitude.
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