[[abstract]]The purposes of this study were to investigate the effects of long-term isokinetic strength training on the levels of cytokine which contains Interleukin 1β(IL-1β),Tumor necrosis factorα(TNF-α), Interleukin-6(IL-6), and Interferones g (IFN-g). The study further extended to investigate the differences of acute on cytokine separately after strenuous isokinetic strength test were taken. Thirty-eight university male students who had not received any special training was recruited as the subjects for this study. They were divided into three different groups: eccentric, concentric and controls. The eccentric and concentric groups had received 18 times (3 d/wk; 6 wk) isokinetic strength training. Blood samples were taken from antecubital vein. Cytokine concentration in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The results were concluded as the following. 1. There were no significant difference of the effect on the concentration of IL-1β and TNF-α after the acute isokinetic strength test were taken.The concentration of IL-6 and IFN-g secreted by leukocyte were reduced, but only IFN-g decreased significantly, (t=-2.06*,p<0.05), after the intensive isokinetic strength test . 2. The isokinetic strength test made two different reactions types on the cytokine to different results. The ability of leukocyte’s secretion apparently decreased on the high reaction of IL-6 and IFN-g type group (IL-6 t=-2.11*,p<0.05; IFN-g t=-2,44*,p<0.05). However, the ability of leukocyte’s secretion was increased , but only IL-6 made significant difference (IL-6 t=2.70*,p<0.95; IFN-g t=1.81, p>0.05) on the low reaction of IL-6 and IFN-g type group. 3. The effects of cytokine levels from the intensive isokinetic strength tests on the first and the second time (after three weeks) were compared separately, and found the effect of the second time was more significant than that of on the first time, and TNF-α showed apparently different(F=9.80,p<0.05). 4. There were no significant difference on the levels of the cytokine, IL-1β, TNF-α, IL-6, and IFN-g, after concentric and eccentric training were taken for 6 wk separately. It was concluded that the maximal isokinetic strength test of single violent one made a slight effect on IL-1β,TNF-α, and IL-6, but it made a restraint on IFN-g, it is probable that the subject possessed different reactions of their leukocyte secretion. The concentrations of cytokine on the second violent exercise stimulation was lower than that on the first time. The 6 wk isokinetic strength training, either concentric or eccentric, made no significantly effect on the concentration of IL-1β, TNF-α, IL-6, and IFN-g . keywords：Isokinetic strength training ; Eccentric training; Concentric training; Cytokine; Interleukin-1β(IL-1β); Tumor Necrosis Factor-α(TNF-α); Interleukin-6 (IL-6) Interferon g (INF- g)
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