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[[alternative]]Study and Application of Poly(vinylidene fluoride) in piezoelectric Bio-Immunosensor

By [[author]]陳姿穎, [[author]]Chen Tsu-Ying, 陳姿穎 and Chen Tsu-Ying

Abstract

[[abstract]]The PVDF coated piezoelectric quartz crystal sensor was employed to study the interaction between PVDF and Proteins. The partially irreversible responses for these proteins were observed by the desorption study, which implied these proteins could be immobilized onto PVDF coated PZ crystals and proteins immobilized onto a phase of PVDF was better than onto a+g phase of PVDF. Proteins were immobilized onto nonpolar a phase of PVDF by hydrophobic interaction. The antibody (anti-human IgG) immobilization on a phase of PVDF was applied in a piezoelectric crystal immunosensor for human IgG in water. The immobilized antibody was still actived that could be used to detect human IgG in water. The antibody immobilization was influenced by PVDF coating load and anti-IgG concentration; The optimum PVDF coating load was found to be 1.1mg with 0.025mg/mL anti-IgG. The strong interaction between IgG and anti-IgG was found, and thus Glycine-HCl could be used to desorb IgG form Anti-IgG. The piezoelectric immuno-sensor could bw reused for human IgG with good reproducibility. The lifetime of immobilized PVDF/anti-human IgG was about 6 days at 4℃. The interaction of anti-human IgG and human IgG was influenced by pH, temperature, and IgG concentration. Optimum pH and optimum temperature were found to be 7.2 and 35℃ respectively. The detection limit of the sensor for IgG was found to be 146.7 ng/mL. The interaction of anti-human IgG and human IgG affinity in organic solvents such as acetone, propylamin, propyl aldehyde, propyl acid was studied. The interference of ions (e.g. Cd2+, Mn2+, Zn2+, Co2+) were also investigated and discussed. Furthermore, the interaction of PVDF and metallic compound were also investigated. PVDF films, filled with various of transition metal compounds, were prepared. The effects of crystalline and electronic structural variations were investigated by infrared analysis. The b+g phase PVDF films could be prepared by dissolving PVDF in 80% acetone+20% DMSO. The b-phase PVDF could be obtained by dissolving PVDF in 80% acetone+20% DMSO with copper salts, e.g., Copper(Ⅱ) Sulfate and Copper(Ⅱ) nitrate. The IR spectra were employed to detect PVDF with 16 transition metal ions. Among these metal ions the addition of Cadmium(Ⅱ) nitrate resulted in the greatest b-phase content PVDF. The interaction between PVDF and transition metal ions was proposed to be electrostatic attraction, and the doping of metal ions with higher charges (e.g., Nb5+andCr3+) could result in higher b-phase content. The effect of radius of metal ions was also found the formation of b-phase of PVDF. The third transition metals with quite big radii gave low content of b-phase PVDF. In contrast, the doping of Cd(Ⅱ) result in quite high content of b-phase PVDF which could be attributed to and the Cd(Ⅱ) ion can be filled in the space between two fluorine atoms quite well. Owing to the presence of transition metal ion would increase b-phase content of PVDF, so a polar and piezoelectricity PVDF film could be simply prepared.

Topics: 聚偏二氟亞乙烯, 壓電生化感測器, 免疫球蛋白G, IR光譜, Poly(vinylidene fluoride), piezoelectric Bio-Immunosensor, IgG, IR spectra, [[classification]]39
Year: 2010
OAI identifier: oai:ir.lib.ntnu.edu.tw:309250000Q/2705
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