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Recalage spatio-temporel de séquences d'images de microscopie 3D de méristèmes floraux d'Arabidopsis

By Gaël Michelin, Yassin Refahi, Raymond Wightman, Henrik Jönsson, Jan Traas, Christophe Godin and Grégoire Malandain

Abstract

International audienceThe shoot apical meristem (SAM) is at the origin of all the plant above-ground organs (including stems, leaves and flowers) and is a biological object of interest for the understanding of plant morphogenesis. The quantification of tissue growth at a cellular level requires the analysis of 3D microscopic image sequences of developing meristems. To address inter-individual variability, it is also required to compare individuals. This obviously implies the ability to process inter-individual registration, i.e. to compute spatial and temporal correspondences between sequences from different meristems.In the present work, we propose a spatial registration method dedicated to microscopy floral meristem (FM) images, and the identification, for a given still image of a meristem, of its best corresponding time-point in a sequence of an other individual (temporal registration)

Topics: Confocal microscopy, developmental biology, floral meristem, Arabidopsis thaliana, [INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV], [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis
Publisher: HAL CCSD
Year: 2016
DOI identifier: 10.1109/ISBI.2015.7163872
OAI identifier: oai:HAL:hal-01606687v2
Provided by: HAL-Inserm
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