Skip to main content
Article thumbnail
Location of Repository

Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site

By Joen Luirink, Jon Beckwith, Gregory Koningstein, Edwin van Bloois, Dirk-Jan Scheffers, Gert Jan Haan, Carine Robichon and Tanneke den Blaauwen

Abstract

The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.

Year: 2007
OAI identifier: oai:ub.rug.nl:dbi/5052f06163c4e
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://gbb.eldoc.ub.rug.nl/roo... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.