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Real-time visualization of intracellular hydrodynamics in single living cells

By Eric O. Potma, Wim P. de Boeij, Peter J.M. van Haastert and Douwe A. Wiersma

Abstract

Intracellular water concentrations in single living cells were visualized by nonlinear coherent anti-Stokes Raman scattering (CARS) microscopy. In combination with isotopic exchange measurements, CARS microscopy allowed the real-time observation of transient intracellular hydrodynamics at a high spatial resolution. Studies of the hydrodynamics in the microorganism Dictyostelium discoideum indicated the presence of a microscopic region near the plasma membrane where the mobility of water molecules is severely restricted. Modeling the transient hydrodynamics eventuated in the determination of cell-specific cytosolic diffusion and plasma membrane permeability constants. Our experiments demonstrate that CARS microscopy offers an invaluable tool for probing single-cell water dynamics.

Year: 2001
OAI identifier: oai:ub.rug.nl:dbi/46d6a7c522ac0
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