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Protonmotive-force-driven leucine uptake in yeast plasma membrane vesicles

By Miroslava Opekarovà, Arnold J.M. Driessen and Wil N. Konings


Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A Δψ of -109 mV was generated in the sealed vesicles by a valinomycin-mediated potassium diffusion potential. This component of protonmotive force was able to drive a transient uptake of leucine.

Year: 1987
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