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Plant U13 orthologues and orphan snoRNAs identified by RNomics of RNA from Arabidopsis nucleoli

By Sang Hyon Kim, Mark Spensley, Seung Kook Choi, Cristiane Calixto, Ali Pendle, Olga Koroleva, Peter J. Shaw and John W. S. Brown

Abstract

Small nucleolar RNAs (snoRNAs) and small Cajal body-specific RNAs (scaRNAs) are non-coding RNAs whose main function in eukaryotes is to guide the modification of nucleotides in ribosomal and spliceosomal small nuclear RNAs, respectively. Full-length sequences of Arabidopsis snoRNAs and scaRNAs have been obtained from cDNA libraries of capped and uncapped small RNAs using RNA from isolated nucleoli from Arabidopsis cell cultures. We have identified 31 novel snoRNA genes (9 box C/D and 22 box H/ACA) and 15 new variants of previously described snoRNAs. Three related capped snoRNAs with a distinct gene organization and structure were identified as orthologues of animal U13snoRNAs. In addition, eight of the novel genes had no complementarity to rRNAs or snRNAs and are therefore putative orphan snoRNAs potentially reflecting wider functions for these RNAs. The nucleolar localization of a number of the snoRNAs and the localization to nuclear bodies of two putative scaRNAs was confirmed by in situ hybridization. The majority of the novel snoRNA genes were found in new gene clusters or as part of previously described clusters. These results expand the repertoire of Arabidopsis snoRNAs to 188 snoRNA genes with 294 gene variants

Publisher: Oxford University Press
Year: 2010
OAI identifier: oai:centaur.reading.ac.uk:4480

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Citations

  1. (2007). A dynamic scaffold of pre-snoRNP factors facilitates human box C/D snoRNP assembly.
  2. (2001). A small nucleolar guide RNA functions both in 2’-O-ribose methylation and pseudouridylation of the U5 spliceosomal RNA.
  3. (2002). Biogenesis of small nucleolar ribonucleoproteins.
  4. (2007). Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs.
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