Skip to main content
Article thumbnail
Location of Repository

Molecular genetic differentiation in earthworms inhabiting a heterogeneous Pb-polluted landscape

By J Andre, R A King, S R Stürzenbaum, P Kille, Mark Edward Hodson and A J Morgan

Abstract

A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages ('A' and 'B'), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central 'ecological island' site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies

Topics: 500
Publisher: Elsevier
Year: 2010
OAI identifier: oai:centaur.reading.ac.uk:4353

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.