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Identification of a novel type of spacer element required for imprinting in fission yeast

By Suha Sayrac, Sonya Vengrova, Emma L. Godfrey and Jacob Z. Dalgaard


Asymmetrical segregation of differentiated sister chromatids is thought to be important for cellular differentiation in higher\ud eukaryotes. Similarly, in fission yeast, cellular differentiation involves the asymmetrical segregation of a chromosomal\ud imprint. This imprint has been shown to consist of two ribonucleotides that are incorporated into the DNA during laggingstrand\ud synthesis in response to a replication pause, but the underlying mechanism remains unknown. Here we present key\ud novel discoveries important for unravelling this process. Our data show that cis-acting sequences within the mat1 cassette\ud mediate pausing of replication forks at the proximity of the imprinting site, and the results suggest that this pause dictates\ud specific priming at the position of imprinting in a sequence-independent manner. Also, we identify a novel type of cis-acting\ud spacer region important for the imprinting process that affects where subsequent primers are put down after the\ud replication fork is released from the pause. Thus, our data suggest that the imprint is formed by ligation of a not-fullyprocessed\ud Okazaki fragment to the subsequent fragment. The presented work addresses how differentiated sister\ud chromatids are established during DNA replication through the involvement of replication barriers

Topics: QH301
Publisher: Public Library of Science
Year: 2011
OAI identifier: oai:wrap.warwick.ac.uk:34580

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  4. (1990). A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. doi
  5. (1998). A site- and strand-specific DNA break confers asymmetric switching potential in fission yeast. doi
  6. (1987). Cell lineage asymmetry for Schizosaccharomyces pombe: Unilateral transmission of a high-frequency state of mating-type switching in diploid pedigrees. doi
  7. (1983). Cell type switching by DNA transposition in fission yeast. doi
  8. (1987). Differentiated parental DNA strands confer developmental asymmetry on daughter cells in fission yeast. doi
  9. (1993). DNA polymerase-alpha is essential for mating-type switching in fission yeast. doi
  10. (2006). DNA primase acts as a molecular brake in DNA replication. doi
  11. (2003). Early-replicating heterochromatin.
  12. (1994). Efficient synthesis of 32P-labeled single-stranded DNA probes using linear PCR; application of the method for analysis of strand-specific DNA repair. doi
  13. (2000). Fission yeast switches mating type by a replication-recombination coupled process. doi
  14. (2004). Formation, maintenance and consequences of the imprint at the mating-type locus in fission yeast. doi
  15. (1988). Four mating-type genes control sexual differentiation in the fission yeast.
  16. (1984). Genes required for initiation and resolution steps of mating-type switching in fission yeast. doi
  17. (1989). Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing.
  18. (1981). Mode of conjugation in homotallic cells of Schizosaccharomyces pombe. doi
  19. (2005). Molecular and cellular dissection of mating-type switching steps in Schizosaccharomyces pombe. doi
  20. (1991). Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. doi
  21. (2008). Mus81 is essential for sister chromatid recombination at broken replication forks. doi
  22. (1990). Optimizing the northern blot procedure.
  23. (1999). Orientation of DNA replication establishes matingtype switching pattern in S. pombe.
  24. (2009). Random and site-specific replication termination. doi
  25. (1997). Replication initiation point mapping. doi
  26. (2004). RNase-sensitive DNA modification(s) initiates S. pombe mating-type switching. doi
  27. (1994). Sap1, a protein that binds to sequences required for mating-type switching, is essential for viability in Schizosaccharomyces pombe.
  28. (2007). Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. doi
  29. (1969). Sinsheimer RL
  30. (2008). Stem cell identity and template DNA strand segregation. doi
  31. (2004). Swi1 and Swi3 are components of a replication fork protection complex in fission yeast. doi
  32. (2000). swi1 and swi3 perform imprinting, pausing, and termination of DNA replication doi
  33. (2003). Swi1 prevents replication fork collapse and controls checkpoint kinase Cds1. doi
  34. (1990). The developmental fate of fission yeast cells is determined by the pattern of inheritance of parental and grandparental DNA strands. doi
  35. (1987). The in vivo replication origin of the yeast 2 microns plasmid. doi
  36. (1987). The localization of replication origins doi
  37. (1993). The mechanism of fission yeast mating-type interconversion: evidence for two types of epigenetically inherited chromosomal imprinted events. doi
  38. (1984). The pedigree pattern of mating-type switching in Schizosaccharomyces pombe. doi
  39. (2005). The Schizosaccharomyces pombe imprint—nick or ribonucleotide(s)? Curr Biol : - 15: R326–327; author reply R327. doi
  40. (1993). The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion. doi
  41. (2006). The wild-type Schizosaccharomyces pombe mat1 imprint consists of two ribonucleotides. doi
  42. (1998). Vectors for the expression of tagged proteins doi

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