Location of Repository

Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers

By Luciana do Valle Rego Oliveira, Ricardo Tadeu de Faria, Claudete de Fátima Ruas, Paulo Maurício Ruas, Melissa de Oliveira Santos and Valdemar P. Carvalho


In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.<br>Neste trabalho, marcadores moleculares de RAPD foram utilizados para acessar a variabilidade genética e estudar as relações interespecíficas e intraespecífica em um grupo de 37 espécies, compreendendo 56 plantas individuais. Um total de 15 primers foram selecionados para amplificação do DNA. De um total de 221 bandas analisadas, 209 (95%) foram polimórficas. O nível de similaridade genética interespecífica variou de 37% entre Catasetum complanatum e Catasetum laminatums a 83% entre Catasetum triodon e Catasetum uncatum. A similaridade genética intraespecífica variou de 88% entre os indivíduos de Catasetum triodon a 93% entre os indivíduos de Catasetum atratum e Catasetum macrocarpum. Os resultados deste trabalho contribuem para o entendimento das relações interespecíficas no gênero Catasetum, para definir estratégias para o estabelecimento de um banco de germoplasma e para dar suporte a programas de melhoramento

Topics: Catasetum, molecular marker, orchid, RAPD, genetic similarity, Biotechnology, TP248.13-248.65, Chemical technology, TP1-1185, Technology, T, DOAJ:Biotechnology, DOAJ:Life Sciences, DOAJ:Biology and Life Sciences
Publisher: Instituto de Tecnologia do Paraná (Tecpar)
Year: 2010
DOI identifier: 10.1590/S1516-89132010000200017
OAI identifier: oai:doaj.org/article:9c7b12b312a94ffebc0a5ca9cae935a4
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1678-4324 (external link)
  • https://doaj.org/toc/1516-8913 (external link)
  • http://www.scielo.br/scielo.ph... (external link)
  • https://doaj.org/article/9c7b1... (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.