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<em>Acalypha indica</em> root extract increases post-hypoxic rat hippocampal tissue culture cell viability via phospholipase A<sub>2</sub> inhibition

By Sophie Yolanda, Trinovita Andraini and Indra Kusuma


<p><strong>Background:</strong> Phospholipase A<sub>2</sub> (PLA<sub>2</sub>) is involved in inflammation and cell death following stroke, and inhibition of its activity may promote neuroregeneration. This study aimed to observe the influence of <em>Acalypha indica</em> Linn root extract towards relative cell viability and PLA<sub>2</sub> enzyme level in post-hypoxic hippocampal tissue culture.</p><p><strong>Methods:</strong> Experimental <em>in vitro</em> study using 24 primary neuronal cell cultures obtained from <em>Sprague Dawley</em> rat exposed to hypoxia with 5% O<sub>2</sub> / 5% CO<sub>2</sub> / N<sub>2</sub> balanced gas for 24 hours. Post-hypoxia, <em>Acalypha indica</em> Linn root extract was added at doses of 10, 15, and 20 mg/mL to three treatment groups. No treatment was given to the control group. Each group consists of six samples. After 72 hours of incubation, relative cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) examination, and phospholipase A<sub>2</sub> enzyme level was determined using ELISA.</p><p><strong>Results:</strong> PLA<sub>2</sub> enzyme level of rat hippocampal tissue culture treated with <em>Acalypha indica</em> Linn root extract at 10, 15, and 20 mg/mL were significantly lower than that of control (5.55 ng/mL, 6.85 ng/mL, and 7.42 ng/mL vs 7.96 ng/mL, p &lt; 0.05).</p><p><strong>Conclusion:</strong> <em>Acalypha indica</em> Linn root extract increases the relative cell viability and decreases the PLA<sub>2</sub> enzyme level of post-hypoxic mouse hippocampal tissue with the optimal dose of the extract at 10 mg/mL. <em><strong>(Med J Indones. 2013;22:136-40 doi: 10.13181/mji.v22i3.581)</strong></em></p><p><strong>Keywords:</strong> <em>Acalypha indica Linn, cell viability, hypoxia, neurogenesis, phospholipase A<sub>2</sub></em></p

Topics: Medicine (General), R5-920, Medicine, R
Publisher: Faculty of Medicine Universitas Indonesia
Year: 2013
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