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Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India

By Mafruza S Rahman, RK Sharma, P Borah, A Chakraborty, Mandakini RK Devi and N Longjam

Abstract

Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding alpha (α) toxin. Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding α toxin

Topics: Clostridium perfringens, PCR, α toxin, cpa gene, polymerase chain reaction, Arctic medicine. Tropical medicine, RC955-962, Special situations and conditions, RC952-1245, Internal medicine, RC31-1245, Medicine, R, DOAJ:Internal medicine, DOAJ:Medicine (General), DOAJ:Health Sciences, Zoology, QL1-991, Science, Q, DOAJ:Zoology, DOAJ:Biology, DOAJ:Biology and Life Sciences, Toxicology. Poisons, RA1190-1270
Publisher: BioMed Central
Year: 2012
DOI identifier: 10.1590/S1678-91992012000100010
OAI identifier: oai:doaj.org/article:04a5940a9e3747cd91924fd2112ad8fd
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