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Validation of PCR Assay for Identification of Sarcoptes Scabiei Var. hominis

By Shumaila Naz, Dilwar ABBas Rizvi, Amara Javaid, Muhammad Ismail and Farhana Riaz Chaudhry

Abstract

Background: Infestation of the skin by the “itch mite” Sarcoptes scabiei var. hominis results in a contagious skin infection in humans called “sca­bies”. By resolving morphology issues, the present study was designed to be acquainted with itch mite by molecular markers.Methods: The mite samples were collected from scabies patients by visiting government hospitals of twin City, Pakistan. For successful molecu­lar detection approach, preparation of Sarcoptes mite DNA by commer­cial DNA extraction kit method. Furthermore, two primers i.e. Sarms 15 F/R and 16S D1/D2 were used to amplify target sequence by using PCR. The amplified products were then separated by agarose gel, electrophoresis and analyzed after staining and visualizing in UV transillu­minator.Results: Analysis of PCR product showed one specific band of 178 bp with primer Sarms 15 F/R, while, with primer 16S D1/D2 bands of 460 bp and 600 bp were observed on 2% agarose gel. The appearance of different band of 600 bp revealed that it might be due to heteroplasmy state present in the Pakistani Sarcoptes mites population.Conclusion: Current study adds validity to the claim that PCR is more accurate, specific and sensitive in the detection of the ectoparasites even in smallest amount

Topics: Sarcoptes Scabiei, PCR Technique, DNA Extraction, Itch Mite DNA, Infectious and parasitic diseases, RC109-216, Internal medicine, RC31-1245, Medicine, R, DOAJ:Internal medicine, DOAJ:Medicine (General), DOAJ:Health Sciences
Publisher: Tehran University of Medical Sciences
Year: 2013
OAI identifier: oai:doaj.org/article:f2eae0775d42449d801126292270d774
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