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Mechanism of transcription factor Twist on promoting peritoneal fibrosis in patients undergoing peritoneal dialysis

By Zhen JIA, Li-hua JI, Jing-he CHEN, Wei LIANG, Ming-wen CHE, Li-jie HE, Shi-ren SUN and Han-min WANG

Abstract

Objective To explore the mechanism of transcription factor Twist on promoting peritoneal fibrosis of human peritoneal mesothelial cells (HPMCs) in patients undergoing peritoneal dialysis (PD). Methods The HPMCs in peritoneal fluid after dialysis from patients underwent peritoneal dialysis was collected and cultured. The expression and subcellular localization of E-cadherin, α-SMA, Twist and Bmi-1 were assayed by immunofluorescence and Western blotting. The expression of Twist, Bmi-1, E-cadherin and α-SMA were determined by immunofluorescence and Western blotting after the plasmids pcDNA3.1-twist and empty vector pcDNA3.1 were transfected into HPMCs with Lipofectamine 2000 in vitro. Twist, Bmi-1, E-cadherin and α-SMA were determined by Western blotting and immunofluorescence after silencing the expression of Twist by small interfering RNA (siRNA) and empty vector pSlience. Bmi-1, E-cadherin and α-SMA were assessed by Western blotting and immunofluorescence after silencing the expression of Bmi-1 by siRNA. Results The expression of E-cadherin decreased gradually and the expression of Twist, α-SMA and Bmi-1 increased along with the increase in PD time as determined with immunofluorescence and Western blotting. Compared with that expressed in the HPMCs trasfected with empty vector pcDNA3.1, the expression of E-cadherin significantly reduced and the expressions of α-SMA, Twist and Bmi-1 increased significantly after transfection of the plasmids pcDNA3.1-twist (P<0.05). The expression of E-cadherin increased significantly and the expressions of α-SMA, Twist and Bmi-1 decreased significantly after silencing the expression of twist by siRNA. The expression of E-cadherin increased significantly and the expressions of α-SMA and Bmi-1 decreased significantly after silencing the expression Bmi-1 by siRNA. Conclusion Twist may be involved in the process of peritoneal fibrosis, and its underlying mechanism is the promotion of the transdifferentiation of HPMCs via the regulation of Bmi-1. DOI: 10.11855/j.issn.0577-7402.2013.11.00

Topics: peritoneal fibrosis, epithelial-to-mesenchymal transition, Twist transcription factor, Bmi-1, cadherin, actins, Medicine (General), R5-920, Medicine, R, DOAJ:Medicine (General), DOAJ:Health Sciences
Publisher: Editorial Board of Medical Journal of Chinese People's Liberation Army
Year: 2013
OAI identifier: oai:doaj.org/article:fe3b0239f8d04b729a9bea39c4118e9c
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