Skip to main content
Article thumbnail
Location of Repository

Microflow Cytometers with Integrated Hydrodynamic Focusing

By Martin Schmidt, Nicole Bock, Hülya Ragusch, Peter Simon, Andreas Kummrow, Janko Theisen, Marcin Frankowski and Jörg Neukammer

Abstract

This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample—A suspension of micro particles or blood cells—is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood

Topics: microfluidics, flow cytometry, lab-on-a-chip, single cell analysis, blood cell differentiation, hydrodynamic focusing, ultraprecision milling, Technology (General), T1-995, Technology, T, DOAJ:Technology (General), DOAJ:Technology and Engineering, Analytical chemistry, QD71-142, Chemistry, QD1-999, Science, Q, DOAJ:Analytical Chemistry, DOAJ:Chemistry, Chemical technology, TP1-1185
Publisher: MDPI AG
Year: 2013
DOI identifier: 10.3390/s130404674
OAI identifier: oai:doaj.org/article:0da13722a9aa4e23bbf2c09811bd39a2
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1424-8220 (external link)
  • http://www.mdpi.com/1424-8220/... (external link)
  • https://doaj.org/article/0da13... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.