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The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses

By Ward Mandy J, Go Alvin C, Li Jun and Ottemann Karen M

Abstract

<p>Abstract</p> <p>Background</p> <p>Chemical-in-plug assays are commonly used to study bacterial chemotaxis, sometimes in the absence of stringent controls.</p> <p>Results</p> <p>We report that non-chemotactic and non-motile mutants in two distinct bacterial species (<it>Shewanella oneidensis </it>and <it>Helicobacter pylori</it>) show apparent zones of accumulation or clearing around test plugs containing potential attractants or repellents, respectively.</p> <p>Conclusions</p> <p>Our results suggest that the chemical-in-plug assay should be used with caution, that non-motile or non-chemotactic mutants should be employed as controls, and that results should be confirmed with other types of assays.</p

Topics: Medicine (General), R5-920, Medicine, R, DOAJ:Medicine (General), DOAJ:Health Sciences, Biology (General), QH301-705.5, Science, Q, DOAJ:Biology, DOAJ:Biology and Life Sciences
Publisher: BioMed Central
Year: 2010
DOI identifier: 10.1186/1756-0500-3-77
OAI identifier: oai:doaj.org/article:1f19cc6342104544b322f2cc1a14cbc0
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