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Fecal specimens preparation methods for PCR diagnosis of human taeniosis

By Nunes Cáris Maroni, Lima Luiz Gustavo Ferraz, Manoel Camila Santos, Pereira Rodrigo Norberto, Nakano Mauro Massaharu and Garcia José Fernando

Abstract

Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®

Topics: Taenia saginata, DNA extraction, Polymerase chain reaction (PCR), Fecal specimens, Medicine (General), R5-920, Medicine, R, DOAJ:Medicine (General), DOAJ:Health Sciences, Arctic medicine. Tropical medicine, RC955-962
Publisher: Universidade de São Paulo
Year: 2006
OAI identifier: oai:doaj.org/article:3911e36c9e274875ba0669b7121e8955
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