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Carbon sources effect on pectinase production from Aspergillus japonicus 586

By Teixeira Maria F. S., Lima Filho José L. and Durán Nelson

Abstract

The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly affects the pectinesterase, and endo- and exopolygalacturonase activities. Pectin, glucose and saccharose, when added to the culture medium in high concentrations, exhibited a repression effect on all the analyzed enzymes

Topics: pectinases, Aspergillus japonicus, enzyme, galacturonase, pectinesterases, Microbiology, QR1-502, Science, Q, DOAJ:Microbiology, DOAJ:Biology, DOAJ:Biology and Life Sciences
Publisher: Sociedade Brasileira de Microbiologia
Year: 2000
OAI identifier: oai:doaj.org/article:3c8896821bbd4a80b6675cb3b1d35ca1
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