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Glass bead transformation method for gram-positive bacteria

By Pongsak Rattanachaikunsopon and Parichat Phumkhachorn

Abstract

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved

Topics: transformation, Gram-positive bacteria, glass bead, Microbiology, QR1-502, Science, Q, DOAJ:Microbiology, DOAJ:Biology, DOAJ:Biology and Life Sciences
Publisher: Sociedade Brasileira de Microbiologia
Year: 2009
DOI identifier: 10.1590/S1517-83822009000400025
OAI identifier: oai:doaj.org/article:52c12d36e52943a69f404363d24e1822
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