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Assessment of the genotoxicity of Cu and Zn in raw and anaerobically digested slurry with the Vicia faba micronucleus test

By Claire-Emmanuelle Marcato, Eric Pinelli, Bertrand Pourrut, Jérôme Silvestre and Agnès Maritchù Guiresse


Genotoxicity of Cu and Zn was assessed by use of the micronucleus (MN) test on Vicia faba roots. Plants were exposed to various leachates of rawand anaerobically digested pig slurry, with maximum total concentrations of 200MCu and 600MZn. The results indicated stabilisation of the organic matter during anaerobic digestion of the slurry and bioconversion of some phytotoxic organic compounds (e.g. phenols or p-cresol), but did not showa relationship between Cu and Zn concentrations and MN frequency. Exposure of Vicia plants to binary inorganic solutions of Cu and Zn (CuSO4/ZnSO4, 1:3) showed a significant micronucleus induction at concentrations of 40M Cu and 120M Zn and higher. When MN frequency was plotted against dissolved Cu (<0.45m), applied as slurry or as CuSO4, a single curve was obtained. At concentrations lower than 10M, modulation of the genotoxic effect of Cu was found. At concentrations up to 150M,MNinduction increased significantly, while phytotoxic symptoms appeared at higher concentrations

Topics: Ecologie, Environnement, Phytotoxicity, Leachates, Metals, Oxidative stress, Organic matter, Bioconversion
Publisher: 'Elsevier BV'
Year: 2009
DOI identifier: 10.1016/j.mrgentox.2008.10.018
OAI identifier:

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  1. (2004). A new approach to evaluating the toxicity and genotoxicity of disinfected drinking water, Water Res. doi
  2. (2003). An inventory of heavy metal inputs to agricultural soils in England and Wales, Sci. Total Environ. 311 doi
  3. (2000). Anaerobic digestion. Holistic bioprocessing of animal manures. In:
  4. (1999). Assessment of the genotoxicity of contaminated soil with the Allium/Vicia- micronucleus and the Tradescantiamicronucleus assays, doi
  5. (2007). Assessment of the genotoxicity of olive mill waste water (OMWW) with the Vicia faba micronucleus test, doi
  6. (1999). Assessment of toxic interactions of heavy metals in binary mixtures: a statistical approach, doi
  7. (1997). Behaviour of metals following intensive pig slurry applications to a natural field treatment process in Brittany (France), doi
  8. (2007). C.E.Marcato,P.Pinelli,P.Pouech,A.Alric,M.Guiresse,Macroandmicronutrients in anaerobically digested pig slurry: recovery of Ca and Mg-Phosphates and nutrient fate in soil, in:
  9. (1999). C.Q.Duan,B.Hu,X.H.Jiang,C.H.Wen,Z.Wang,Y.X.Wang,Genotoxicityofwater samples from Dianchi lake detected by the Vicia faba micronucleus test, doi
  10. (2006). Copper-inducedchangesinthegrowth, oxidative metabolism, and saponin production in suspension culture roots of Panax ginseng in bioreactors, doi
  11. (1987). D.A.Scicchitano,A.E.Pegg,InhibitionofO6-alkylguanine-DNA-alkyltransferase by metals, doi
  12. (1990). Determination of mass balances and ionic balances in animal manure,
  13. (2003). Dietary means to better control the environmental impact of copper and zinc by pigs from weaning to slaughter, doi
  14. (2003). Distribution and bioavailability of copper in farm effluent, Sci. Total Environ. doi
  15. (1999). Effects of anaerobic digestion and additives to effluent or cattle feed on odor and odorant concentrations,
  16. (1996). Effects of composting on phytotoxicity of spent pig-manure sawdust litter, doi
  17. (2000). Evaluation of toxic and genotoxic activity of some composts from different origin,
  18. (1998). Genotoxic effects of heavy metals: comparative investigation with plant bioassays, doi
  19. (2002). H.B.Møller,S.G.Sommer,B.K.Ahring,Separationefficiencyandparticlesizedistribution in relation to manure type and storage conditions, doi
  20. (1990). How to characterize a biological antioxidant, Free Rad.
  21. (2001). J.A.Diez,A.I.delaTorre,M.C.Cartagena,M.Carballo,A.Vallejo,M.J.Mu˜ noz,Evaluation of the application of pig slurry to an experimental crop using agronomic and ecotoxicological approaches, doi
  22. (1995). Met calfe, Fish micronuclei for assessing genotoxicity in water, doi
  23. (2000). Mu˜ noz, Ecotoxicological evaluation of pig slurry, doi
  24. (2004). Mutagens in contaminated soil: a review, doi
  25. (2002). Panda Genotoxicity and mutagenicity of metals in plants. doi
  26. (2008). Particle size and metal distributions in anaerobically digested pig slurry, doi
  27. (2005). Plant-specific responses to zinc contamination in a semi-field lysimeter and on hydroponics, doi
  28. (2006). Reduction of malodorous compounds from a treated swine anaerobic lagoon, doi
  29. (2005). Study on the toxicity of binary equitoxic mixtures of metals using the luminescent bacteria Vibrio fischeri as a biological target, doi
  30. (2004). T90-327. Soil quality – assessment of genotoxic effects to higher plants, in:
  31. (1985). The effects of anaerobic digestion on heavy metal distribution in sewage sludge,
  32. (1996). The Genotoxicity of Zn(II) and Cd(II) in Allium cepa Root Meristematic Cells, New Phytol. doi
  33. (1995). The improved Allium/Vicia root tip assay for clastogenicity of environmental pollutants, doi
  34. (2004). The mutagenic hazards of aquatic sediments: a review, doi
  35. (2005). Treatment with biosolids affects soil availability and plant uptake of potentially toxic elements, doi
  36. (2004). V.P.Utgikar,N.Chaudhary,A.Koeniger,H.H.Tabak,J.R.Haines,R.Govin,Toxicity of metals and metal mixtures: analysis of concentration and time dependence for zinc and copper, Water Res. doi
  37. (1999). Vicia root micronucleus assay on the clastogenicity of water samples from the Xiaoqing River in Shandong Province of the People’s Republic of China, doi

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