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Wound response in orange as a resistance mechanism against <i>Penicillium digitatum</i> (pathogen) and <i>P. expansum</i> (non-host pathogen)

By L. Vilanova, Rosario Torres, I. Viñas, Luis González Candelas, Josep Usall i Rodié, Stefano Fiori, Cristina Solsona and N. Teixidó

Abstract

<i>Penicillium digitatum</i> is the most devastating postharvest pathogen of citrus. In addition, <i>Penicillium expansum</i> is the main pathogen of pome fruit, although recent studies have demonstrated its ability to infect oranges under some conditions. In this study, we evaluated wound response in ‘Valencia’ oranges harvested at three different maturity stages and the effect of wound response on the establishment of both pathogens when fruit were stored at two different temperatures (20 and 4 °C). The effect of wounding and pathogen inoculation on lignin content, was also quantified. Lastly, the expression of several phenylpropanoid pathway-related genes was also analyzed by semi-quantitative RT-PCR. Results indicated that, in general, <i>P. digitatum</i> exhibited lower decay incidence and severity as time between wounding and inoculation increased. Decay incidence and severity were higher in fruit from the over-mature harvest than in fruit from immature and commercial harvests. <i>P. expansum</i> was able to infect fruit at 20 °C but lesions were small compared to lesion size of fruit stored at 4 °C. Lignin content in wounded fruit (control) and in samples wounded and inoculated with <i>P. expansum</i> was highest in fruit from the immature harvest at 7 d post-wounding and inoculation. Wounded fruit had higher expression of <i>pal1</i>, <i>comt1</i> and <i>pox1</i> genes at 48 h than at 24 h. However, samples inoculated with <i>P. digitatum</i> showed lower expression at 48 h than at 24 h. Our results indicated that maturity and storage temperature play an important role in orange wound response

Topics: AGR/12 Patologia vegetale
Publisher: Elsevier
Year: 2013
OAI identifier: oai:eprints.uniss.it:8721
Provided by: UnissResearch
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