Article thumbnail
Location of Repository

Application of an unusual ninhydrin-based reaction for the indirect chiral resolution of D,L-penicillamine

By Salvatore Sotgia, Angelo Zinellu, Gérard Aimé Pinna, Luca Deiana and Ciriaco Carru


An unusual reaction involving ninhydrin and aminothiols was exploited to set an indirect method for the chiral recognition of stereoisomers of penicillamine. Separation of diastereoisomers was achieved on a C18 column in isocratic mode by using a mixture of propionic acid (pH 3.0)/acetonitrile/water (10:10:80, v/v/v) as a mobile phase. Diastereoisomers were detected by a fluorescence detector in fairly short times (about 7 min) and with a good resolution. The lowest detectable amount of toxic isomer of penicillamine (l-enantiomer) in samples of the d-enantiomer, was around 0.01%. The method was also suitable for the indirect chiral recognition of other aminothiols such as cysteine and cysteinylglycine

Topics: BIO/14 Farmacologia, BIO/12 Biochimica clinica e biologia molecolare clinica
Publisher: Elsevier
Year: 2011
DOI identifier: 10.1016/j.talanta.2011.07.007
OAI identifier:
Provided by: UnissResearch
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.