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Deep UV sensing of the interaction of porphyrin with bovine serum albumin protein

By Q Li and S Seeger


The interaction of meso-tetrakis(p-sulfonatophenyl)porphyrin (TPPS) with bovine serum albumin (BSA) in neutral solution (pH 7.4) has been studied by means of absorption, steady-state fluorescence and time-resolved fluorescence spectroscopy. The formation of TPPS–BSA complex was monitored by spectroscopic characteristic changes in Soret band absorption and fluorescence emission of TPPS. Applying the time-correlated single-photon counting (TCSPC) method, BSA–TPPS interaction was also investigated by the fluorescence lifetime of tryptophan residues in BSA. The results demonstrate that deep UV laser-based fluorescence lifetime microscopy is useful for sensitive identification of protein interaction using intrinsic fluorescence

Topics: Department of Chemistry, 540 Chemistry
Publisher: Elsevier
Year: 2009
DOI identifier: 10.1016/j.snb.2008.10.001
OAI identifier:
Provided by: ZORA

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