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STUDY OF THE REGENERATION OF AUTOTRANSPLANTED SPLENIC TISSUE IN THE RABBIT

By ΔΗΜΗΤΡΗΣ ΧΑΡΑΛΑΜΠΙΔΗΣ

Abstract

THE PURPOSE OF THIS STUDY WAS TO STUDY THE REGENERATION OF THE AUTOTRANSPLANTEDSPLENIC TISSUE IN THE RABBIT, AND ESTABLISH THE FUNCTIONAL STATUS OF THE AUTOTRANSPLANTED ORGANISM. 65 ADULT FEMALE NEW ZEALAND WHITE RABBITS WERE DIVIDED IN FOUR GROUPS: NORMAL CONTROLS (A), SPLENECTOMISED (B), AND AUTOTRANSPLANTED (C&D). WE STUDIED THE ABILITY OF THE POLYMORPHONUCLEAR LEUCOCYTES (PMN) FORPHAGOCYTOSIS, INTRACELLULAR KILLING, AND PRODUCTION OF IMMUNOGLOBULINS. ON THE 12TH WEEK THE ANIMAL UNDERWENT A MORTALITY TEST BY TRANSTRACHEAL INOCULATION OF PNEUMOCOCCUS. ALL ANIMALS UNDERWENT POST-MORTEM AND THE REGENERATED AUTOTRANSPLANTED SPLEEN WAS STUDIED AND COMPARED WITH THE NORMAL. THE LUNGS, LIVER AND ADRENAL WERE ALSO STUDIED HISTOLOGICALLY. THE TEST REVEALED THE FOLLOWING CONCLUSIONS. 1- SPLENECTOMY DELAYS THE PHAGOCYTIC ABILITY OF THE PMN BY DECREASING A PLASMA FACTOR, BUT DOES NOT AFFECT THE ABILITY OF THE PMN. 2- THE AUTOTRANSPLANTED SPLENIC TISSUE AT 12 WEEKS RESTORES THE ABILITY OF PMN FOR PHAGOCYTOSIS. 3- ABSENCE OF ACTIVE SPLENIC TISSUE RESULTS IN DECREASING RATE OF INTRACELLULAR KILLING BY THE PMN AND 4- THE AUTOTRANSPLANTED SPLENIC TISSUERESTORES THIS ABILITY. 5- SPLENECTOMY RESULTS IN DECREASING THE LEVELS OF IGM AND IGG, WHERE AUTOTRANSPLANTATION INCREASES BOTH IMMUNOGLOBULINS BUT FAILED TO RESTORE THEM TO NORMAL LEVELS. 6- REMOVAL OF THE SPLEEN MAKES THE HOST SUSCEPTIBLE TO PNEUMOCOCCAL INFECTIONS. (ABSTRACT TRUNCATED)

Topics: AUTOTRANSPLANATION, Immunoglobulins, INDUCED RESPIRATORY SEPSIS, INTRACELLULAR KILLING, Phagocytocis, Splenectomy, Ανοσοσφαιρίνες, ΑΥΤΟΜΕΤΑΜΟΣΧΕΥΣΗ, ΕΝΔΟΚΥΤΤΑΡΙΟΣ ΘΑΝΑΤΟΣ, ΠΡΟΚΛΗΤΗ ΜΙΚΡΟΒΙΑΙΜΙΑ, Σπληνεκτομή, Φαγοκυττάρωση
Publisher: University of Crete (UOC)
Year: 1996
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