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The ClC-7 Chloride Channel Is Downregulated by Hypoosmotic

By Stress In Human Chondrocytes


Articular chondrocytes in osteoarthritis (OA) patients are exposed to hypoosmotic stress because the osmolality of this synovial fluid is significantly decreased. Hypoosmotic stress can cause an efflux of Cl2 and an associated decrease of cell volume. We have previously reported that a Cl2 conductance contributes to the regulation of resting membrane potential and thus can alter intracellular Ca21 concentration ([Ca21]i) in human chondro-cytes. The molecular identity and pathologic function of these Cl2 channels, however, remained to be determined. Here, we show that the ClC-7 Cl2 channel is strongly expressed in a human chondrocyte cell line (OUMS-27) and that it is responsible for Cl2 currents that are activated by extracellular acidification (pH 5.0). These acid-sensitive currents are inhibited by 4,4ʹ-diisothiocyanatostilbene-2,2ʹ-disulfonic acid (DIDS; IC505 13mM

Year: 2014
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