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Purified CD34+ Lin- Thy+ Stem Cells Do Not Contain Clonal Myeloma Cells

By Yair Gazitt, Chris C. Reading, Ron Hoffman, Amittha Wickrema, David H. Vesole, Sundar Jagannath, Janet Condino, Brenda Lee, Bart Barlogie and Guido Tricot


High-dose therapy with autologous marrow or peripheral blood stem cell (PBSC) rescue has been extensively applied in the treatment of multiple myeloma (MM) patients during the past 10 years resulting in improved event-free and over-all survival when compared with standard chemotherapy. However, relapses are common and cure is unlikely in the majority of patients. Because both bone marrow and PBSCs are contaminated with myeloma cells it is conceivable that relapse after autotransplantation originates at least in part from autografted tumor cells. In this study, mobilized PBSCs were examined for the presence of myeloma cells based on immunophenotyping and sensitive polymerase chain reac-tion (PCR)-based techniques. In addition, CD34+ Lin- Thy+ stem cells were purified from obilized PBSC harvests of 10 MM patients by sequentially using counterflow elutriatio

Year: 2016
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