Magnetic Cell Separation for Elimination of Corneal Stromal Fibroblast Contamination in


Human corneal endothelial cells (HCECs) are responsible for the major water-pumping function of the cornea, from corneal stroma to the aqueous humor, and any damage to these cells may lead to corneal decompensation. When the endothelium functions adequately, it regulates stromal hydration and thus both corneal thickness and transparency which are critical for optimal function. This function is accomplished by an ATP-dependent endothelial pump function, in addition to the presence of focal tight junctions which act as a barrier. HCEC density decreases with aging and with various diseases such as bullous keratopathy and Fuchs ’ endothelial dystrophy. The conventiona

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