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The intestinal microbiota interferes with the microRNA response upon oral Listeria infection

By Cristel Archambaud, C Odile Sismeiro, D Joern Toedling, H Guillaume Soubigou, D Christophe Bécavin, C Pierre Lechat, Alice Lebreton, C Constance Ciaudo and H Pascale Cossarta


ABSTRACT The intestinal tract is the largest reservoir of microbes in the human body. The intestinal microbiota is thought to be able to modulate alterations of the gut induced by enteropathogens, thereby maintaining homeostasis. Listeria monocytogenes is the agent of listeriosis, an infection transmitted to humans upon ingestion of contaminated food. Crossing of the intestinal bar-rier is a critical step of the infection before dissemination into deeper organs. Here, we investigated the role of the intestinal mi-crobiota in the regulation of host protein-coding genes andmicroRNA (miRNA or miR) expression during Listeria infection. We first established the intestinal miRNA signatures corresponding to the 10 most highly expressed miRNAs in the murine ileum of conventional and germfree mice, noninfected and infected with Listeria. Next, we identified 6 miRNAs whose expression de-creased upon Listeria infection in conventional mice. Strikingly, five of these miRNA expression variations (in miR-143, miR-148a, miR-200b, miR-200c, andmiR-378) were dependent on the presence of the microbiota. In addition, as is already known, protein-coding genes were highly affected by infection in both conventional and germfree mice. By crossing bioinformatically the predicted targets of the miRNAs to our whole-genome transcriptomic data, we revealed anmiRNA-mRNA network that sug-gested miRNA-mediated global regulation during intestinal infection. Other recent studies have revealed anmiRNA response t

Year: 2013
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