Abstract

13. PBMCs from each donor were plated at 5 3 105 cells per well in 96-well microtiter plate in 200 ml of RPMI 1640 medium containing penicillin (100 U/ml), streptomycin (0.1 mg/ml), 2 mM glutamine, 1 mM sodium pyruvate, 10 mM Hepes, and antigen (10 mg/ml) in triplicate. During the last 20 hours of the 72-hour culture period, 1 mCi of [3H]thymidine was added per well. Incorporation was measured by liq-uid scintillation counting