THE NUTRITIONAL REQUIREMENTS OF CLOSTRIDIUM PARABOTULINUM, A

Abstract

This investigation was undertaken with the purpose of de-veloping a synthetic medium for Clostridium parabotulinum, type A, in order to study certain phases of the toxin-producing mechanism. The results mainly confirmed the findings of Fildes and his co-workers (1933) with respect to the essential nature of certain substances in yeast and pregnancy urines. In addition, certain evidence is presented which indicates that other substances besides the "sporogenes vitamin " are required for growth of C. parabotulinum in amino acid mixtures. The nature of these materials will be the subject of subsequent notes. Studies on the simplification of media for the cultivation of Clostridium botulinum have not often been the primary purpose of researches on this organism. In fact, it is only in recent year