Article thumbnail
Location of Repository

AN ABSTRACT OF THE THESIS OF ASRIL DARUSSAMIN for the degree of DOCTOR OF PHILOSOPHY in BIOCHEMISTRY AND BIOPHYSICS presented on December 20,1984 Title: REACTIONS OF HORSERADISH PEROXIDASE WITH THE ISOTHIOCYANATES OF HORSERADISH OIL

By 

Abstract

Horseradish peroxidase (HRP) has been investigated extensively and for many years. In spite of this it still exhibits unexplained anomalies. Among these is variation in the reported number of isozymes (from 7 to 42), and reports of instability of the isozyme patterns. Since horseradish is a rich source of organic isothiocya-nates (mustard oils), it seemed likely that artifactual chemical modification of the enzyme might occur during or after extraction. The present investigation demonstrates that isothiocyanates can in fact cause extensive modifi-cation of HRP without corresponding loss of catalytic activity. Incubation of HRP with isothiocyanates at alkaline pH and 4°C altered the isozyme pattern. Peroxidase activity was shifted from bands of higher p1 to bands o

Year: 2016
OAI identifier: oai:CiteSeerX.psu:10.1.1.957.7992
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://ir.library.oregonstate.... (external link)
  • http://ir.library.oregonstate.... (external link)
  • http://citeseerx.ist.psu.edu/v... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.