Mutant SI/SP mice exhibit decreased marrow hematopoie-sis. The defect is known t o reside in the marrow microenviron-ment of these animals, which is reproduced in vitro by primary marrow explants as well as by cloned marrow stromal cell lines. Bone marrow progenitor cells are incapa-ble of adhering to primary Sl/Sp stromal cells or cloned stromal cell lines derived from them to form cobblestone-islands and proliferate. The role of hemonectin, a marrow-specific adhesion protein in the defective hematopoiesis of the Sl/Sp mice, was studied. Indirect immunoperoxidase staining of marrow in situ from SIISP mice showed little specific staining while specific staining was seen in a pericel-lular distribution in marrow from +/+ mice. Hemonectin expression in several cloned stromal cell lines from SIISP mice was compared by immunoblotting with that in clone
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