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Evaluation of multiple sewage-associated bacteroides PCR markers for sewage pollution tracking

By Warish Ahmed, Ashantha Goonetilleke, Daniel Powell and Ted Gardner

Abstract

The host specificity of the five published sewage-associated Bacteroides markers (i.e., HF183, BacHum, HuBac, BacH and Human-Bac) was evaluated in Southeast Queensland, Australia by testing fecal DNA samples (n = 186) from 11 animal species including human fecal samples collected via influent to a sewage treatment plant (STP). All human fecal samples (n = 50) were positive for all five markers indicating 100% sensitivity of these markers. The overall specificity of the HF183 markers to differentiate between humans and animals was 99%. The specificities of the BacHum and BacH markers were > 94%, suggesting that these markers are suitable for sewage pollution in environmental waters in Australia. The BacHum (i.e., 63% specificity) and Human-Bac (i.e., 79% specificity) markers performed poorly in distinguishing between the sources of human and animal fecal samples. It is recommended that the specificity of the sewage-associated markers must be rigorously tested prior to its application to identify the sources of fecal pollution in environmental waters

Topics: 090702 Environmental Engineering Modelling, 050206 Environmental Monitoring, Fecal pollution, Water quality, Microbial source tracking, Bacteroides, Sewage-associated markers
Publisher: Elsevier
Year: 2009
DOI identifier: 10.1016/j.watres.2009.08.042
OAI identifier: oai:eprints.qut.edu.au:29217

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