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Native type II collagen-induced arthritis in the rat. I. Incidence and humoral response to collagen. Ann Rheum Dis

By K. Morgan, R. B. Clague, Mary J. Shaw and P. J. L. Holt


SUMMARY An acute inflammatory arthritis has been induced in 76 % of rats injected intradermally with native bovine type II collagen emulsified in Freund's complete (CFA) or incomplete (ICFA) adjuvant. The arthritis became chronic in 14 out of 31 rats, and ear and tail lesions were noted in some rats. No arthritis was induced by native type I collagen, denatured type II collagen, rabbit IgG, or buffer alone injected intradermally with adjuvant. Using a solid-phase radioimmunoassay for serum antibodies we have shown that IgM and IgG levels to native bovine type II collagen were significantly higher in arthritic than nonarthritic rats. Antiglobulin antibody levels were not raised in arthritic rats. Equivalent antibody levels to native type II collagen were obtained whether this antigen was emulsified in ICFA or CFA. However, native type I collagen produced high antibody levels only when emulsified in CFA, indicating a difference in the immunogenicity of these collagens. These studies suggest that native type II collagen possesses arthritogenic properties in the rat and that humoral immunity may play a role in the induction of this arthritis. The induction of a chronic inflammatory arthritis in the rat by the intradermal injection of human, chick, or rat collagen obtained from articular carti-lage and emulsified in either Freund's complete (CFA) or incomplete (1CFA) adjuvant has recently been described by Trentham et al. (1977). The incidence of arthritis was approximately 40 % in the rat strains tested and mainly affected the hind limbs. Both cellular and humoral immune responses to native type II collagen can be detected in these rats, and there appears to be some correlation between higher levels of sensitivity to the collagen and the presence of arthritis (Trentham et al., 1978). We here describe the induction and clinical course of arthritis in the rat using a different hetero-logous native type II collagen (bovine) and the humoral responses in arthritic and nonarthritic rats. Materials and methods Rats. Outbred female Sprague-Dawley rats (150

Topics: 250 g) from the Manchester Medical School Closed
Year: 1980
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