BRIEF COMMUNICATION Histological Sectioning of the Rodent Larynx for


In rodents, the larynx is a major site of histopathologic alteration following inhalation exposure to partic-ulates, vapors, and aerosols. Specifically, the epithelial lining of a narrowly delineated region on the ventral floor of the larynges of rats and mice appears to be especially vulnerable to inhaled materials, and is recognized as a preferred site for histopathological evaluation in inhalation studies. This site is located at the base of the epiglottis, cranial to the ventral laryngeal diverticulum (ventral pouch). The presence of underlying seromucinous glands is critical for histologic identification of this site. We report a histologic sectioning technique, using the ventral laryngeal diverticulum as the anatomical landmark, to obtain tissue sections from this area of predilection in rats and in mice. Keywords. Larynx; histology; inhalation toxicology; serial sectioning; epithelium The larynx is an organ of considerable interest in inhalation toxicity studies in rats and mice (2, 7). A narrowly delineated region of ventral laryngeal epithelium is highly responsive to inhaled materials such as cobalt sulfate (1) and tobacco smoke in bot

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