Preliminary Structural Analysis of L1 1-Pyrroline-5-carboxylate


Mangrove plants differ in an ability growing in high salt concentration from any other plants. On the mechanism of their salt tolerance, there are many physiological and morphological reports so far, but few reports on the mechanism at molecular level. In some mangrove species, proline is accumulated in cells in response to salt stress I). Proline can be synthesized by a pathway from glutamate in higher plants under the condition of salt stress. The key enzyme in the pathway is Ll I-pyrroline-5-carboxylate synthetase (P5CS) which catalyzes the first two steps of proline biosynthesis2,3). In this paper, we describe the cloning and sequence analysis of P5CS gene (BgP5CS) fragments isolated from mangrove (Bruguiera gymnorrhiza) genome by polymerase chain reaction (PCR). Four grams of mangrove leaves (B. gymnorrhiza) were pulverized with liquid nitrogen to fine poweder. Genomic DNA was extracted from the pulverized tissue with CTAB (Cetyltrimethl ammonium bromide) extraction solutio

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