Genotyping of Polymorphisms in AlcoholandAldehyde Dehydrogenase Genes by DirectApplication of PCR-RELP on Dried Blood withoutDNA Extraction


We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genoiypinu method was used to successfully and Tenably genotypeADHIB andALDH2 polymorphisms without DNA isolation using a 12-min disc of dried blood and the KOD FX PCR enzyme kit. SNP genotyping was performed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. In addition 10 the labor and expense advantages, the possibility of sample contamination was considerably decreased, since the DNA extraction step was eliminated. In the post-genome era, a simple and inexpensive method for diagnostic analysis is in high demand, and this method will be very useful for genetic diagnoses in biolouical and medical laboratorie

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