Use of a microsome-mediated test system to assess efficacy and mechanisms of cancer chemopreventive agents


2To whom correspondence should be addressed There is a growing need for short-term assays which can assess the mechanisms and efficacy of cancer chemopreven-tive agents. In the present study we have employed a microsome-mediated test system concomitantly with DNA adduct detection to assess the efficacy of five chemopreven-tive agents, iV-acetylcysteine, butylated hydroxytoluene (BHT), curcumin, oltipraz, and ellagic acid. 32P-Postla-beling analysis of DNA incubated with benzo[a]pyrene (BP) in the presence of Aroclor 1254-induced microsomes produced two major adducts: one derived from the inter-action of benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) with deoxyguanosine (dG) and the other from further activation of 9-OH-BP (309 and 34 adducts/107 nucleotides, respect-ively). With the exception of N-acetylcysteine, all test agent

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