Rapid Detection of Carbapenemaseproducing Pseudomonas spp


A novel biochemical technique, the Carba NP test, has been evaluated to detect carbapenemase production in Pseudomonas spp. This test was specific (100%), sensitive (94.4%), and rapid (<2 h). This cost-effective test, which could be implemented in any microbiology laboratory, offers a reliable technique for identification of carbapenemase-producing Pseudomonas spp. Pseudomonas aeruginosa is intrinsically resistant to a number of-lactamsdue to the lowpermeability of its outermembrane, the constitutive expression of various efflux pumps, and the production of -lactamases (5). Acquired resistance to broad-spectrum -lac-tams is increasinglyobserved inP.aeruginosa.Currently,PER-,VEB-, and GES-type enzymes are the most frequently observed extended-spectrum -lactamases (ESBLs) identified in Pseudomonas spp. (5, 7). Therefore, carbapenems are considered crucial for treating many P. aeruginosa-associated infections. In Pseudomonas spp., carbapenem resistance may be related either to a decreased bacterial outer membrane permeability (e.g., loss or modification of the OprD2 porin or overexpression of efflux pumps), often associatedwith overexpression of-lactama-ses possessing no significant carbapenemase activity (AmpCs), or to expression of true carbapenemases (5, 14). In Pseudomonas spp., those carbapenemases are mostly metallo--lactamases (MBLs) of the VIM, IMP, SPM, GIM, AIM, DIM, andNDM types and, to a lesser extent, Ambler class A carbapenemases of the KP

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