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Changes in the intestinal microbiota after a short period of dietary over-indulgence, representative of a holiday or festival season

By Christos Gougoulias, Sanoja Sandaradura, Xiao Meng, Anne C. Perz, Anthony R. Leeds and Linda V. Thomas


The effects on the intestinal microbiota of a short period of marginal over-eating, characteristic of holiday or\ud festival periods, were investigated in a pilot study. Fourteen healthy male subjects consumed a diet rich in\ud animal protein and fat for seven days. During this period, the subjects significantly increased their dietary\ud energy, protein, carbohydrate and fat intakes by 56, 59, 53 and 58%, respectively (all P < 0.05). The mean\ud weight gain of 0.27 kg was less than the expected 1 kg, but this was consistent with a degree of under-reporting\ud on the baseline diet. Fluorescence in situ hybridisation analysis confirmed the relative stability of each\ud individual’s faecal microbiota but showed considerable variations between them. The diet was associated with\ud a significant increase in numbers of total faecal bacteria and the bacteroides group, as detected by the universal\ud bacterial probe (DAPI) and Bacteroides probe (Bac 303), respectively. Overall, there was a decrease in\ud numbers of the Lactobacillus/Enterococcus group (Lab 158 probe; 2.8 ± 3.0% to 1.8 ± 1.8%) and the Bifidobacterium\ud group (Bif 164 probe; 3.0 ± 3.7% to 1.7 ± 1.2%), although there was considerable inter-individual\ud variation. Analysis of the relative proportions of each bacterial group as a percentage of the subject’s total\ud bacteria showed a trend for a change in the intestinal microbiota that might be considered potentially\ud unhealthy

Publisher: IFIS
Year: 2008
OAI identifier:

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  23. (2005). Gut microorganisms, mammalian metabolism and personalized health care.
  24. (2003). Honor thy symbionts. doi
  25. (1978). Influence of diet and age on fecal bacterial enzymes.
  26. (1989). Influence of pH, nutrient availability and growth rate on amine production by Bacteroides fragilis and Clostridium perfringens.
  27. (1988). Inhibition of secondary bile acid formation in the large intestine by lactulose in healthy subjects of two different age groups.
  28. (2002). Meat consumption and colorectal cancer risk: dose-response metaanalysis of epidemiologic studies.
  29. (2005). Medix Intelligent Information.
  30. (1992). Metabolic response to experimental overfeeding in lean and overweight healthy volunteers.
  31. (1997). N-nitroso compounds and man: sources of exposure, endogenous formation and occurrence in body fluids.
  32. (2000). Phylogenetic relationships of butyrate-producing bacteria from the human gut.
  33. (1995). Preventive effect of a Lactobacillus casei preparation on the recurrence of superficial bladder cancer in a double blind trial.
  34. (2000). Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization.
  35. (1994). Regulatory effects of bifidobacteria on the growth of other colonic bacteria.
  36. (2007). Research Fund/American Institute for Cancer Research. doi
  37. (1993). Suppressing effect of Lactobacillus casei administration on the urinary mutagenicity arising from ingestion of fried ground beef in the human.
  38. (2000). Systematic review of epidemiological studies on meat, dairy products and egg consumption and risk of colorectal adenoma.
  39. (2001). Systematic review of the prospective cohort studies on meat consumption and colorectal cancer risk: a meta-analytical approach.
  40. (1998). Temperature gradient gel electrophoresis analysis from human fecal samples reveals stable and host-specific communities of active bacteria.
  41. (1992). The Christmas factor in obesity therapy.
  42. (1998). The effect of consumption of milk fermented by Lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans.
  43. (1989). The effect of diet on the intestinal microflora. In:
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