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Study on the effect of green pit viper venom (Trimeresurus albolabris) on platelet morphology by flow cytometry

By Suphan Soogarun, Montri Choewbamrungkiat, Viroj Wiwanitkit, Jamsai Suwansaksri, Waykin Nopanitaya, Paweena Pradniwat, Attakorn Palasuwan and Supantitra Chanprasert


The incidence of venomous snake bites, especially by green pit viper, has been increasing every year in Thailand. The bite of green pit viper causes bleeding because of thrombin–like property of the venom and it has been reported that the mean platelet volume decreases in those bitten by this snake. The objective of this study was to study the effect of green pit viper venom (Trimeresurus albolabris) on platelet volume (MPV), number of platelets and platelet morphology in vitro. The test was carried out by washing platelet in phosphate buffer pH 7.2 so as to get rid of fibrinogen, then the washed platelets were mixed with green pit viper venom. The mean platelet volume and number of platelets were determined by flow cytometry. The results showed that there was a decrease in the number of platelets (216±101 x 109/l and 78.1±43.4 x 109/l; P< 0.05) and also the MPV (8.9±1.2fl and 4.8±1.3fl, P< 0.05). The platelet size was smaller than normal, ranging from 1.1-1.2 micrometers. In conclusion, the green pit viper venom had a direct effect on platelet morphology, especially by decreasing platelet volume and numbers

Topics: Online Journal of Health and Allied Sciences
Publisher: Dr. B.S. Kakkilaya
Year: 2006
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