indirect correlation with cAMP/cGMP ratio

Abstract

Background: CD24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. Our aim was to clarify the correlation between CD24 expression and cAMP:cGMP ratio in murine colorectal cancer cell line (CT26) after using cholera toxin. Materials and Methods: The CT26 cells were cultured in flasks for assaying cAMP and cGMP by ELISA kits; also the cells were cultured in flasks for assaying cytoplasmic and membranous CD24 expression. The Real-Time PCR was done for cDNA that was synthesized from CT26 cells ’ mRNA. Also, expression CD24 marker of cells was determined by Anti-CD24 antibody and Goat Anti-Rabbit IgG-FITC (flow cytometry). Results: The cholera toxin increases the cAMP:cGMP ratio and influenced the cytoplasmic and membrane CD24 expression level. Conclusion: Cholera toxin increased cAMP. After increasing cAMP/cGMP ratio, cytoplasmic and membranous CD24 expression showed decline