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NOTES Purification and Characterization of a New Heat-Stable Enterotoxin Produced by Vibrio cholerae Non-O1 Serogroup Hakata

By Michiko Arita, Takeshi Honda, Toshio Miwatani and L Katsuhiko Ohmori


The possible production of a heat-stable enterotoxin (Vc-H-ST) by Vibrio cholerae non-Ol serogroup Hakata was investigated, and the purified Vc-H-ST was characterized. It has a unique amino acid sequence, LIDCCEICCNPACFGCLN. This sequence is quite similar to that of the heat-stable enterotoxin (NAG-ST) produced by V. cholerae non-Ol except for one amino acid (leucine) residue excess at the N terminus. Other characteristics, including biological activity, are compatible with those of NAG-ST. Vibrio cholerae non-Ol serogroup Hakata, often isolated from the environment and from imported seafood in Japan, is a new serotype of V. cholerae non-O1 proposed by Shimada et al. (7). V. cholerae non-Ol serogroup Hakata is agglutinated with polyvalent anti-V. cholerae 01 antisera but not with antisera against the A antigen (factor) that is a common antigen among V. cholerae 01 (7). This sometimes causes misidentification of V. cholerae non-Ol serogroup Hakata as V. cholerae 01. In this study, the possible production of a heat-stabl

Year: 1990
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