Skip to main content
Article thumbnail
Location of Repository

Identification of caspase 3 motifs and critical aspartate residues in human Phospholipase D1b and Phopsholipase D2a

By Michelle H. Wright, Michelle J. Farquhar, Mina-Olga Aletrari, Graham Ladds and Matthew N. Hodgkin


Stimulation of mammalian cells frequently initiates phospholipase D-catalysed\ud hydrolysis of phosphatidylcholine in the plasma membrane to yield phosphatidic acid\ud (PA) a novel lipid messenger. PA plays a regulatory role in important cellular\ud processes such as secretion, cellular shape change and movement. A number of\ud studies have highlighted that PLD-based signalling also plays a pro-mitogenic and\ud pro-survival role in cells and therefore anti-apoptotic. We show that human PLD1b\ud and PLD2a contain functional caspase-3 cleavage sites and identify the critical\ud aspartate residues within PLD1b that affect its activation by phorbol esters and\ud attenuate phosphatidylcholine hydrolysis during apoptosis

Topics: R1
Publisher: Elsevier
Year: 2008
OAI identifier:

Suggested articles


  1. (2003). A decade of Caspases. doi
  2. (1996). A novel family of phospholipase d homologues that includes phospholipid synthases and putative endonucleases: Identification of duplicated repeats and potential active sites. doi
  3. (2002). Anti-apoptotic role of phospholipase D in spontaneous and delayed apoptosis of human neutrophils. doi
  4. (2002). Antigen stimulated activation of phospholipase D1b by doi
  5. (1998). Association of N-and C-terminal domains of phospholipase D1 is required for its catalytic activity.
  6. (2004). Calphostin-C induction of vascular smooth muscle cell apoptosis proceeds through phospholipase D and microtubule inhibition. doi
  7. (2007). Caspase Substrates.
  8. (2002). Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells. doi
  9. (1998). Decreased phospholipase D activity in ceramide-induced apoptosis of human keratinocyte cell line HaCaT. doi
  10. (2002). Differential effects of Fas crosslinking on phospholipase D activation and related lipid metabolism in Fas-resistant A20 cells. doi
  11. (2003). Evolutionary conservation of physical and functional interactions between phospholipase D and actin. doi
  12. (1998). Increased activity of oleate-dependent type phospholipase D during actinomycin D-induced apoptosis in Jurkat T cells.
  13. (1997). Involvement of caspase-dependent activation of cytosolic phospholipase A2 in tumor necrosis factor-induced apoptosis. doi
  14. (2004). Koda M,Hara A and Nozawa Y doi
  15. (1999). Mammalian caspases: structure, activation, substrates and functions during apoptosis. Annual Review in doi
  16. (2003). Many cuts to ruin: a comprehensive review of caspase susbtrates. doi
  17. (2006). Phopholipid signalling through phospholipase D and phosphatidic acid. doi
  18. (2001). Phosphatidic acid and diacylglycerol directly activate NADPH oxidase by interacting with enzyme components. doi
  19. (2002). Phospholipase C-protein kinase c mediated phospholipase D activation pathway is involved in tamoxifen-induced apoptosis. doi
  20. (2003). Phospholipase D prevents apoptosis in v-Src transformed fibroblasts and MDA MB 231 breast cancer cells. doi
  21. (2000). Phospholipase D regulation and localisation is dependent upon a phosphatidylinositol 4,5-bisphosphate-specific PH domain. doi
  22. (2001). Phospholipase D1 localises to secretory granules and lysosomes and is plasma-membrane translocated on cellular stimulation. doi
  23. (2004). Programmed cell death: apoptosis and alternative death styles.
  24. (2000). Regulation of phospholipase D activity and ceramide production in daunorubicin-induced apoptosis in A-431 cells. doi
  25. (2003). Regulation of phospholipase d1 subcellular cycling through coordination of multiple membrane association motifs.
  26. (2002). Role of Phospholipase D in apoptosis and pro-survival. doi
  27. (2007). Role of phospholipase D2 in anti-apoptotic signaling through increased expressions of Bcl-2 and Bcl-xL. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.