The tribe Acrotylini are considered as pest of cereal crops, vegetables, orchards and pastures in Pakistan. During present study 5 species and subspecies of the tribe Acrotylini i-e Acrotylus humbertianus (Saussure), A. insubricus insubricus (Scopoli) A. patruelis (Herrich-Schaffer) A. longipes longipes (Charpentier) and A. longipes subfasciatus were examined. In this manuscript general morphological characteristic along with detail study of phallic complex of each species has carried out. Overall finding showed that there is significant difference in the epiphallus, ancorae, lophi and spermatheca of these siblings ’ species of Acrotylini

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