oaioai:CiteSeerX.psu:10.1.1.599.3284

Variation in the HindlII Restriction Fragments of DNA from the Chinese

Abstract

HindlII fragments D to P of DNA from a Chinese vaccine strain (Tian Tan) of vaccinia virus have been molecularly cloned into the plasmid pAT153 at the unique HindlII site. The Chinese strain DNA differs from a non-vaccine American strain (WR) in having an additional HindlII fragment (P). Twelve HindlII D clones and 12 HindlII F clones of the Chinese strain were analysed by digestion by EcoRI, BamHI, PstI and XhoI. Two forms of D (designated a and b) and of F (a and b) were demonstrated. In each the differences were detected as the presence of an additional EcoRI site, in Da and Fa. The HindlII Fa and Fb fragments of the Chinese strain were shown to differ significantly from the WR strain in their restriction site maps. In recent years, interest has arisen in the use of the vaccinia virus genome as a eukaryotic expression vector for the construction of live recombinant vaccines directed against both human and veterinary infectious diseases or for the synthesis of biological products. Panicali & Paoletti (1982), Panicali et al. (1983) and Mackett et al. (1982) successfully adapted site-specific recombination between the HindlII F or J fragments of vaccinia virus DNA flanking the foreign DNA and homologous sequences in replicating viral DNA to allow insertion of th

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oaioai:CiteSeerX.psu:10.1.1.599.3284Last time updated on 10/29/2017

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