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Determination of Intracellular pH by ^(31)P Magnetic Resonance

By Richard B. Moon and John H. Richards


Observation of the ^(31)P signal from various intracellular phosphates can provide a convenient, nondestructive technique for determining intracellular conditions such as pH. This procedure has been explored with particular reference to the erythrocyte. Both the chemical shift of intracellular inorganic phosphate relative to that of serum phosphate and the positions of, and more especially the difference between, the chemical shifts of 2,3-diphosphoglycerate have been used to monitor intracellular pH of erythrocytes whose hemoglobin has been liganded with carbon monoxide

Publisher: American Society for Biochemistry and Molecular Biology
Year: 1973
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Provided by: Caltech Authors
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