Skip to main content
Article thumbnail
Location of Repository

Protein-protein interactions involved in baculovirus DNA replication

By 

Abstract

Graduation date: 1998The yeast two-hybrid system was used to examine\ud interactions between the nine proteins involved in baculovirus DNA\ud replication. From the six proteins required for DNA replication,\ud four protein-protein interactions were identified, including an\ud interaction between LEF-1 and LEF-2, LEF-3 and itself, LEF-3 and\ud P143 (Helicase), and an interaction between IE-1 and itself.\ud The replication factors LEF-1 and LEF-2 interacted in both\ud yeast two-hybrid assays and glutathione S-transferase fusion\ud affinity assays. Using the yeast two-hybrid system, we mapped\ud the interaction domain of LEF-2 to amino acids between positions\ud 20 and 60. Deletion analysis of LEF-1 failed to reveal an\ud interaction domain, suggesting that either multiple interaction\ud domains exists or the deletions disrupted secondary structures\ud required for the interaction. All of the deletions which were\ud unable to interact also failed to support significant levels of\ud transient DNA replication, suggesting that this interaction plays a\ud significant role in DNA replication.\ud The baculovirus single-stranded DNA binding protein, LEF-3, interacts with itself in yeast two-hybrid assays and glutathione S-transferase fusion affinity assays. Deletions of LEF-3, which were unable to interact with full length LEF-3, also failed to support transient DNA replication, suggesting that this interaction is required for the proper function of LEF-3. LEF-3 was purified to apparent homogeneity and analyzed by analytical ultracentrifugation, native PAGE and MALDI mass spectrometry, identifying the oligomeric structure of LEF-3 as a homotrimer.\ud In addition to interacting with itself, LEF-3 also interacts with P143 in yeast two-hybrid assays, immunoprecipitation experiments, and co-purification from a single-stranded DNA agarose column. The yeast two-hybrid system was used to map the LEF-3 interaction domain to the N-terminal 165 amino acids of LEF-3. Deletion analysis of P143 failed to reveal a delimited interaction domain. C-terminal deletions of LEF-3 containing amino acids 1 to 165 were unable to interact with full length LEF-3, indicating that the interaction of LEF-3 with itself (trimerization) is not required for the interaction between LEF-3 with P143

Year: 1998
OAI identifier: oai:ir.library.oregonstate.edu:1957/33943
Provided by: ScholarsArchive@OSU

Suggested articles

Citations

  1. (1986). A catalog of viral diseases of insects, mites, and ticks."
  2. (1996). A conserved family of cellular genes related to the baculovirus iap gene and encoding apoptosis inhibitors.
  3. (1997). A functional interaction of ICP8, the herpes simplex virus singlestranded DNA-binding protein, and the helicase-primase complex that is dependent on the presence of the UL8 subunit. doi
  4. (1987). A manual of methods for baculovirus vectors and insect cell culture procedures."
  5. (1997). A mechanism for negative gene regulation in Autographa californica multinucleocapsid nuclear polyhedrosis virus.
  6. (1986). A method for identifying the viral genes required for herpesvirus DNA replication. doi
  7. (1987). A new class of yeast transcriptional activators. doi
  8. (1988). A new superfamily of replicative proteins. doi
  9. (1989). A novel genetic system to detect protein-protein interactions. doi
  10. (1994). A putative non-hr origin of DNA replication in the Hindlll -K fragment of Autographa californica nuclear polyhedrosis virus. doi
  11. (1994). A role for two DNA helicases in the replication of T4 bacteriophage DNA. doi
  12. (1996). A variable region of Anticarsia gemmatalis nuclear polyhedrosis virus contains tandemly repeated DNA sequences. Virus Res. doi
  13. (1996). Abortive infection of the baculovirus Autographa californica nuclear polyhedrosis virus in Sf-9 cells after mutation of the putative DNA helicase gene.
  14. (1981). Alpha amanitin resistant viral RNA synthesis in nuclei isolated from nuclear polyhedrosis virus-infected Heliothis zea larvae and Spodoptera frugiperda cells.
  15. (1993). An apoptosisinhibiting baculovirus gene with a zinc finger-like motif.
  16. (1994). An apoptosisinhibiting gene from a nuclear polyhedrosis virus encoding a polypeptide with Cys/His sequence motifs.
  17. (1996). An Autographa californica nucleopolyhedrovirus lef-2 mutant: Consequences for DNA replication and late gene expression. doi
  18. (1974). An enzootic nuclear polyhedrosis virus of pink shrimp: ultrastructure, prevalence, and enhancement. doi
  19. (1978). Analysis of baculovirus genomes with restriction endonucleases. doi
  20. (1988). Antibodies: A laboratory manual." doi
  21. (1996). Apoptotic suppression by baculovirus P35 involves cleavage by and inhibition of a virus-induced CED-3/ICE-like protease.
  22. (1991). Assessing the multimeric states of proteins: studies using laser desorption mass spectrometry. doi
  23. (1994). Association of origin binding protein and single stranded DNAbinding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo.
  24. (1997). Autographa californica nuclear polyhedrosis virus p143 gene product is a DNAbinding protein. doi
  25. (1977). Autographa californica nuclear polyhedrosis virus: Comparative infectivity of the occluded, alkali-liberated, and nonocciuded forms. doi
  26. (1991). Baculoviridae. Nuclear polyhedrosis viruses. In "Atlas of Invertebrate Viruses"
  27. (1990). Baculovirus diversity and molecular biology. doi
  28. (1992). Baculovirus expression vectors. doi
  29. (1991). Baculovirus gp64 gene expression: Analysis of sequences modulating immediate early transcription and transactivation by 1E-1.
  30. (1981). Baculovirus induction of a DNA polymerase.
  31. (1994). Baculovirus p35 prevents developmentally programmed cell death and rescues a ced-3 mutant in the nematode Caenorhabditis elegans.
  32. (1983). Baculovirus replication: inhibition of Trichoplusia ni multiple nuclear polyhedrosis virus by [E]- 5- (2bromovinyl) -2'- deoxyuridine. doi
  33. (1983). Baculovirus replication: purification and identification of the Trichoplusia ni nuclear polyhedrosis virus-induced DNA polymerase. doi
  34. (1981). Baculovirus replication: stimulation of thymidine kinase and DNA polymerase activities in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. doi
  35. (1982). Baculovirus replication. doi
  36. (1986). Baculovirus transcription in the presence of inhibitors and in nonpermissive Drosophila cells. doi
  37. (1988). Baculoviruses as gene expression vectors. doi
  38. (1995). Biochemical and genetic characterization of a replication protein A dependent DNA helicase from the yeast, Saccharomyces cerevisiae. doi
  39. Calf Thymus DNA helicase F, a replication protein A copurifying enzyme. doi
  40. (1986). Changes in DNA polymerase activities in pupae of the silkworm Bombyx mori after induction with nuclear polyhedrosis virus. doi
  41. (1986). Characterization of 3' to 5' exonuclease associated with DNA polymerase of silkworm nuclear polyhedrosis virus. doi
  42. (1988). Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus. doi
  43. (1987). Characterization of baculovirus p10 synthesis using monoclonal antibodies. doi
  44. (1977). Characterization of shrimp baculovirus.
  45. (1997). Characterization of the interaction between the baculovirus replication factors,
  46. (1992). Characterization of the nucleotide sequence of the Lymantria dispar nuclear polyhedrosis virus DNA polymerase gene region. doi
  47. (1993). Characterization of the replication of plasmids containing hr sequences in baculovirus infected Spodoptera frugiperda cells. doi
  48. (1996). Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors. doi
  49. (1986). Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus.
  50. (1991). Construction of an improved baculovirus insecticide containing an insect-specific toxin gene. doi
  51. (1994). Control of programmed cell death by the baculovirus genes p35 and iap.
  52. (1995). Correlation of twohybrid affinity data with in vitro measurements.
  53. (1994). Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA. doi
  54. (1993). D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA. doi
  55. (1947). Die isolierung des polyeder-virus and die natur der polyeder. doi
  56. (1995). Differential requirements for baculovirus late expression factor genes in two cell lines.
  57. (1996). Dityrosine formation in calmodulin: Cross-linking and polymerization catalyzed by Arthromyces peroxidase. doi
  58. (1991). DNA helicases of Escherichia coli. doi
  59. (1992). DNA Replication." doi
  60. (1997). DNA-Dependent transregulation by 1E1 of Autographa californica nuclear polyhedrosis virus: 1E1 domains required for transactivation and DNA binding.
  61. (1970). Established insect cell line from the cabbage looper, Trichoplusia ni. doi
  62. (1991). Expression of an enhancer-binding protein in insect cells transfected with the Autographa californica nuclear polyhedrosis virus 1E1 gene.
  63. (1989). Expression of foreign genes in insects using baculovirus vectors. doi
  64. (1993). Expression of the baculovirus p35 gene inhibits mammalian neural cell death. doi
  65. (1994). Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene. doi
  66. (1974). Free and occluded virus, similar to baculovirus, in hepatopancreas of pink shrimp. doi
  67. (1994). Functional dissection of the Autographa californica nuclear polyhedrosis enhancer element hr5. doi
  68. (1992). Functional dissection of the Autographa californica nuclear polyhedrosis virus immediate-early transcriptional regulatory1 protein.
  69. (1986). Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-early gene.
  70. (1988). Functional mapping of an AcNPV immediate early gene which augments expression of the 1E-1 trans-activated 39K gene. doi
  71. (1994). Functional mapping of regions of the Autographa californica nuclear polyhedrosis viral genome required for DNA replication. doi
  72. GAL11P: A yeast mutation that potentiates the effect of weak GAL4-derived activators. doi
  73. (1992). Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus. doi
  74. (1994). Granulosis viruses.". Encyclopedia of Virology
  75. (1994). Helicase-primase complex of herpes simplex virus type 1: a mutation in the UL52 subunit abolishes primase activity. doi
  76. (1993). Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV.
  77. (1995). Identification and analysis of a putative origin of DNA replication in the Choristoneura fumiferana multinucleocapsid nuclear polyhedrosis virus genome. doi
  78. (1993). Identification and characterization of a putative origin of DNA replication in the genome of a baculovirus pathogenic for Orgyia pseudotsugata. doi
  79. (1995). Identification and characterization of a second putative origin of DNA replication in a baculovirus of Orgyia pseudotsugata. doi
  80. (1993). Identification and characterization of lef-1, a baculovirus gene involved in late and very late gene expression. doi
  81. (1991). Identification and characterization of the 1E-1 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. doi
  82. (1995). Identification of essential trans-acting regions required for DNA replication of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus: lef-1 is an essential replication gene. doi
  83. (1994). Identification of genes involved in DNA replication of the Autographa californica baculovirus. doi
  84. (1994). Identification of lef-7: A baculovirus gene affecting late gene expression. doi
  85. (1993). Identification of seven putative origins of Autographa californica MNPV DNA replication. doi
  86. (1991). Identification of the very early transcribed baculovirus gene PE-38.
  87. (1997). Identification of two independent transcriptional activation domains in the Autographa californica multicapsid nuclear polyhedrosis virus 1E1 protein.
  88. (1995). Identification, localization, transcription, and sequence analysis of the Choristoneura fumiferana nuclear polyhedrosis virus DNA polymerase gene. doi
  89. (1995). Identification, sequence and transcriptional analysis of lef-3, a gene essential for Orgyia pseudotsugata baculovirus DNA replication. doi
  90. (1990). Identifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus. doi
  91. (1993). Immediate-early baculovirus genes transactivate the p143 gene promoter of Autographa californica nuclear polyhedrosis virus. doi
  92. (1993). In vitro transcription from baculovirus late gene promoters: accurate mRNA initiation by nuclear extracts prepared from infected Spodoptera frugiperda cells.
  93. (1991). In vitro transcription of baculovirus immediate early genes: Accurate mRNA initiation by nuclear extracts from both insect and human cells. doi
  94. (1996). Induction of apoptosis by baculovirus transactivator 1E-1.
  95. (1980). Infectious DNA from Autographa californica nuclear polyhedrosis virus. doi
  96. (1995). Inhibition of ICE family proteases by baculovirus antiapoptotic protein P35. doi
  97. (1995). Inhibition of the Caenorhabditis elegans cell-death protease CED-3 by a CED-3 cleavage site in baculovirus P35 protein. doi
  98. (1996). Initiation of baculovirus DNA replication: Early promoter regions can function as infectiondependent replicating sequences in plasmid-based replication assay.
  99. (1991). Insect paralysis by baculovirus-mediated expression of a mite neurotoxin gene. doi
  100. (1992). Interaction of DNA polymerase a-primase with cellular replication protein A and SV40 T antigen. doi
  101. (1986). Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhances delayed-early gene expression.
  102. (1986). Introduction: Historical perspective, in: The Biology of Baculoviruses.". Vol. I: Biological Properties and Molecular
  103. (1991). Isolation of a DNA helicase from HeLa cells requiring the multisubunit human singlestranded DNA-binding protein for activity.
  104. (1983). Location of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome.
  105. (1993). Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus. doi
  106. (1995). Lymantria dispar nuclear polyhedrosis virus homologous regions: characterization of their ability to function as replication origins.
  107. (1991). Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. doi
  108. (1991). Molecular analysis of a baculovirus regulatory gene. doi
  109. (1989). Molecular cloning: a laboratory manual." doi
  110. (1988). Multiple early transcripts and splicing of the Autographa californica nuclear polyhedrosis virus 1E-1 gene.
  111. (1989). Multiple functions of human single-stranded DNA binding protein in simian virus 40 DNA replication: Single-strand stabilization and stimulation of DNA polymerases a and 8. doi
  112. (1991). Novel regulatory properties of the 1E1 and 1E0 transactivators encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus.
  113. (1994). Nuclear polyhedrosis viruses.". Encyclopedia of Virology
  114. (1987). Nucleotide sequence and temporal expression of a baculovirus regulatory gene.
  115. (1995). Nucleotide sequence and transcriptional analysis of the DNA polymerase gene of Bombyx mori nuclear polyhedrosis virus. doi
  116. (1991). Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. doi
  117. (1994). Nucleotide sequence of the polyhedrin gene region of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus: placement of the virus in lepidopteran nuclear polyhedrosis virus group II. doi
  118. (1980). On the origin of the polyhedral viral protein of the nuclear polyhedrosis virus of Autographa californica." doi
  119. (1984). Phenotypic characterization and physical mapping of a temperature-sensitive mutant of AcMNPV defective in DNA synthesis. doi
  120. (1985). Physical Biochemistry."
  121. (1993). Physical interaction between the herpes simplex virus origin-binding protein and single1 stranded DNA-binding protein ICP8. doi
  122. (1991). Prevention of apoptosis by a baculovirus gene during infection of insect cells. doi
  123. (1983). Production of human beta interferon in insect cells infected with a baculovirus expression vector.
  124. (1984). Production of polyhedrin monoclonal antibodies for distinguishing two Orgyia pseudotsugata baculoviruses.
  125. (1993). Proliferating cell nuclear antigen and P21 are components of multiple cell cycle kinase complexes. doi
  126. (1995). Protease activation during apoptosis: Death by a thousand cuts? doi
  127. (1986). Protein volumes and hydration effects. doi
  128. (1943). Proteins, Amino Acids, and Peptides." doi
  129. (1994). Purification and characterization of bacteriophage T4 gene 59 protein.
  130. (1988). Purification and characterization of replication protein A, a cellular protein required for in vitro replication of simian virus 40 DNA. doi
  131. (1987). Rapid and efficient site-specific mutagenesis without phenotypic selection. doi
  132. (1993). RecQ DNA helicase of Escherichia coli. doi
  133. (1994). Reiterated DNA fragments in defective genomes of Autographa californica nuclear polyhedrosis virus are competent for AcMNPV-dependent DNA replication. doi
  134. Replication of baculovirus DNA. doi
  135. (1995). Replication of Orgyia pseudotsugata baculovirus DNA: le-1 and lef-2 are essential and ie-2, p34 and op-iap are stimulatory genes. Virology 212, doi
  136. (1992). Review article: Baculovirus structural proteins. doi
  137. (1998). Role of baculovirus 1E2 and its RING finger in cell cycle arrest.
  138. (1986). Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. doi
  139. (1996). Sequence, function, and phylogenic analysis of an ascovirus DNA polymerase gene. doi
  140. (1992). Sitespecific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell linespecific effects on virus replication.
  141. (1985). Size and map locations of early transcription products of the Autographa californica nuclear polyhedrosis virus genome. doi
  142. (1996). Species-specific effects of the hcf-1 gene on baculovirus virulence.
  143. (1997). Splicing is required for transactivation by the immediate early gene 1 of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus. doi
  144. (1993). Strategies for the identification of interacting proteins. doi
  145. (1984). Strong and regulated expression of Escherichia coli f3- galactosidase in insect cells with a baculovirus vector.
  146. (1991). Structural comparison of the Autographa californica nuclear polyhedrosis virus-induced RNA polymerase and the three nuclear RNA polymerases from the host, Spodoptera frugiperda. doi
  147. (1996). Structure-function analysis of the Autographa californica multinucleocapsid nuclear polyhedrosis virus homologous region palindromes. doi
  148. (1996). Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes. doi
  149. (1992). Tandemly repeated sequence at the 3' end of the 1E-2 gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element. doi
  150. (1994). Targeted versus non-targeted DNA helicase activity of the RuvA and RuvB proteins of Escherichia coli. doi
  151. (1992). The Autographa californica baculovirus genome: Evidence for multiple replication origins. doi
  152. (1995). The Autographa californica nuclear polyhedrosis virus homologous region 1 a: Identical sequences are essential for DNA replication activity and transcriptional enhancer function. Virology 208, doi
  153. (1994). The Autographa californica nuclear polyhedrosis virus ie2 gene encodes a transcriptional regulator. doi
  154. (1995). The baculovirus P35 protein inhibits fas- and tumor necrosis factor-induced apoptosis. doi
  155. (1997). The baculovirus singlestranded DNA binding protein, LEF-3, forms a homotrimer in solution.
  156. (1995). The baculovirus transactivator 1E1 binds to viral enhancer elements in the absence of insect cell factors.
  157. (1997). The Baculoviruses.". The Viruses doi
  158. (1986). The Biology of Baculoviruses. Boca
  159. (1994). The complete DNA sequence of Autographa califomica nuclear polyhedrosis virus. doi
  160. (1996). The DNA polymerase and helicase genes of a baculovirus of Orgyia pseudotsugata. doi
  161. (1976). The effects of inhibitors of nucleic acid and protein synthesis on the replication of Spodoptera frugiperda nuclear polyhedrosis virus in cultured Spodoptera frugiperda cells. doi
  162. (1977). The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). doi
  163. (1994). The gene 59 protein of bacteriophage T4 modulates the intrinsic and singlestranded DNA-stimulated ATPase activities of gene 41 protein, the replicative DNA helicase.
  164. (1993). The hr5 transcriptional enhancer stimulates early expression from the Autographa californica nuclear polyhedrosis virus genome but is not required for virus replication.
  165. (1995). The lef-3 gene of Autographa californica nuclear polyhedrosis virus encodes a singlestranded DNA-binding protein.
  166. (1988). The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene. doi
  167. (1994). The Purification and Characterization of gene 59 protein from bacteriophage T4.
  168. (1996). The putative LEF-1 proteins from two distinct Choristoneura fumiferana multiple nucleopolyhedroviruses share domain homology to eukaryotic primases genome. doi
  169. (1986). The regulation of baculovirus gene expression. doi
  170. (1993). The retinoblastoma protein associates with the protein phosphatase type catalytic subunit.1 doi
  171. (1995). The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication.
  172. (1993). The structural and functional organization of the Autographa californica nuclear polyhedrosis virus genome: an overview. doi
  173. (1995). The TNFR2-TRAF signalling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins. doi
  174. (1991). The twohybrid system: A method to identify and clone genes for proteins that interact with a protein of interest. doi
  175. (1996). The UL8 subunit of the Herpes Simplex Virus Type-1 DNA helicase primase optimizes utilization of DNA templates covered by the homologous single-strand DNA-binding protein ICP8. doi
  176. (1993). Three baculovirus genes involved in late and very late gene expression: ie-1, ie-n, and lef 2.
  177. (1992). Time-of-flight mass specrometry for the structural anasysis of biological molecules.
  178. (1995). Transcriptional enhancer activity of hr5 requires dual-palindrome half sites that mediate binding of a dimeric form of the baculovirus transregulator 1E-1.
  179. (1980). Transfection of two invertebrate cell lines with DNA of Autographa califomica nuclear polyhedrosis virus. doi
  180. (1980). Transfection with baculovirus DNA. doi
  181. (1991). Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements.
  182. (1988). Trends in the development of baculovirus expression vectors. doi
  183. (1987). Unidirectional digestion with exonuclease III in DNA sequence analysis. doi
  184. (1989). Viral proliferating cell nuclear antigen. doi
  185. (1983). Viral transcription during Autographa californica nuclear polyhedrosis virus infection: A novel RNA polymerase induced in infected Spodoptera frugiperda cells.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.