Skip to main content
Article thumbnail
Location of Repository

Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays

By Karl F. Hoffmann and Jennifer M. Fitzpatrick


Hoffmann, K. F., Fitzpatrick, J. M. (2004). Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays, in S. Melville (ed) Methods in Molecular Biology, Vol. 270: Parasite Genomics Protocols. Humana Press Inc., Totowa, NJ. pp. 219-236DNA microarray platforms represent a functional genomics technology that uses structured information obtained from genomic sequencing efforts as a means to study transcriptional processes in a systematic and high-throughput manner. Specifically in this chapter, we outline the ordered processes involved in large-scale parasite gene expression studies including complementary (cDNA) microarray fabrication, total RNA isolation, cDNA labeling using fluorochromes, and DNA:DNA hybridization. Methods described herein were adapted for the study of schistosome sexual maturation and developmental biology but could be easily modified for the study of any additional parasitological system

Publisher: Springer Nature
Year: 2004
DOI identifier: 10.1385/1592597939
OAI identifier:

Suggested articles


  1. (2001). An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression. doi
  2. (2000). Cell-cycle and developmental regulation of TbRAB31 localisation, a GTP-locked Rab protein from Trypanosoma brucei. doi
  3. (1991). Complementary DNA sequencing: expressed sequence tags and human genome project. doi
  4. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. doi
  5. (2001). Exploring the transcriptome of the malaria sporozoite stage. doi
  6. (2001). Expression microarray hybridization kinetics depend on length of the immobilized DNA but are independent of immobilization substrate. doi
  7. (2000). Functional conservation of Schistosoma mansoni Smads in TGF-beta signaling. doi
  8. (2001). Immunological genomics of Brugia malayi: filarial genes implicated in immune evasion and protective immunity. doi
  9. (2000). Importance of replication in microarray gene expression studies: statistical methods and evidence from repetitive cDNA hybridizations. doi
  10. (1999). Making and reading microarrays. doi
  11. (2001). Manufacturing DNA microarrays of high spot homogeneity and reduced background signal. doi
  12. (1989). Molecular Cloning: A Laboratory Manual. doi
  13. (1999). Options available—from start to finish—for obtaining expression data by microarray. doi
  14. (2001). The guanine protein coupled receptor rhodopsin is developmentally regulated in the free-living stages of Schistosoma mansoni. doi
  15. (2000). Toxoplasma gondii catalase: are there peroxisomes in Toxoplasma? doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.