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Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays

By Karl F. Hoffmann and Jennifer M. Fitzpatrick

Abstract

Hoffmann, K. F., Fitzpatrick, J. M. (2004). Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays, in S. Melville (ed) Methods in Molecular Biology, Vol. 270: Parasite Genomics Protocols. Humana Press Inc., Totowa, NJ. pp. 219-236DNA microarray platforms represent a functional genomics technology that uses structured information obtained from genomic sequencing efforts as a means to study transcriptional processes in a systematic and high-throughput manner. Specifically in this chapter, we outline the ordered processes involved in large-scale parasite gene expression studies including complementary (cDNA) microarray fabrication, total RNA isolation, cDNA labeling using fluorochromes, and DNA:DNA hybridization. Methods described herein were adapted for the study of schistosome sexual maturation and developmental biology but could be easily modified for the study of any additional parasitological system

Publisher: Springer Nature
Year: 2004
DOI identifier: 10.1385/1592597939
OAI identifier: oai:cadair.aber.ac.uk:2160/13136

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